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Enzyme biology coursework

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Enzyme biology coursework

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Effect of Substrate Concentration on the Rate of Activity of Catalase | Owlcation Catalase. Hydrogen Peroxide (2H O) Water (2H O) + Oxygen (O). Introduction: Enzyme is a protein produced by a living organism that acts as. In the preliminary I carried out an experiment which could be used to measure the volume of gas produced, when different concentrations of hydrogen peroxide were catalysed by the enzyme catalase. Analysis From the graph, I can see that as the concentration of hydrogen peroxide decreased, the volume of oxygen produced decreased as a direct result. Average final volumes of oxygen for each concentration of hydrogen peroxide.

Biology Coursework Enzymes


Let us show you how we can help you. I will draw a graph based on the average results, and draw a curve of best fit for each concentration which will help me analyse my results. Place the six conical flasks in a water bath at 25 o C to create a constant external temperature and dissipate heat energy. Competitive inhibitors are inhibitors which form the shape of the substrate that is complementary to the enzyme active site. This means that when ordering for our services you can be sure of writing that is correct and free from errors in sentence structure, spelling, and punctuation. The diagram above shows at a lower concentration there are a lower number of hydrogen peroxide molecules. Note: Use a pipette rather than a conical flask or a measuring cylinder because pipettes are very accurate for measuring volumes. I will make sure that I measure 0. I will work out the gradient of these new results and plot them on a new rate of reaction graph. In my hypothesis I predicted that the rate of reaction would increase with increase of Hydrogen peroxide concentration. This is done by using 10 1 mm potato discs in each experiment. This results in a bigger proportion of molecules having a kinetic energy greater than that of the activation energy. The temperature coefficient Q10 for an enzyme controlled reaction between 0 and 45oC is 2. Without catalase, the decomposition would take much longer and would not be fast enough to sustain human life. In order to make sure that my results are reliable I will be repeating the experiment for all concentrations twice. This is shown in the table with all the results I have gained Fig. Recording Results I will record my results in a table like the one below, and then record further, average results, in a similar table. The gas syringe, however, has a small volume of air displaced within it when it is attached to the conical flask, so I will have to consider this in the main procedure. Most scholars taking biology have a tough time when starting and finishing their coursework. I have explained this later on. Draw a curve of best fit on your graph. I believe that as the concentration of the hydrogen peroxide substrate decreases, the rate of reaction will decrease as well. Contact us today and begin to see the difference. Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time. This is mainly because enzymes have preferable working condition in which they work. Urgent or Specialized Writing Help? This would have allowed me to measure the temperature correctly at which the hydrogen peroxide and the catalse are. Worry no more, your lab experiments will be handled by professionals. Introduction Catalase is an enzyme which is found in most living organisms. This is because as the concentration decreased, the number of molecules of hydrogen peroxide also decreased. Free Essay: Biology Enzymes Coursework Prediction: I think that the enzyme will work best at c to c I think this because that optimum temperature for.


Enzyme biology coursework - Help with Osmosis, Beetroot Experiments and Enzyme Assays


The volume of gas escaping coming from the delivery tube to the burette which will result in an inaccurate reading. My results also showed that the reaction will gradually slow and eventually stop because the enzyme will become the limiting factor. Well, we have a number of reasons why you should trust us with your biology coursework. I would expect this graph to be linear as this would show that as the concentration increases, the time taken for a set volume of gas would decrease. The affect of enzyme concentration is that it will increase the rate of reaction. Our assistants will also guide you through other aspects of your coursework, such as formatting and citation, ensuring that you will always get the value for your money. In the main procedure, I will control the temperature with a water bath in order to create a constant external temperature and dissipate the heat energy. Tags: biology. I carried out a preliminary experiment in order to know how to carry out this experiment. I am experienced in the fields of biology, physics and chemistry, and I enjoy writing on these topics. Another error in the preliminary experiment was that the temperature. We will develop your writing skills and make you a pro in coursework writing. This is used to display charts and graphs on articles and the author center. As result of faster moving particles there will be more frequent collisions between enzymes and substrate which will lead to form more products in a given time. Enzyme is a protein produced by a living organism that acts as a catalyst in a specific reaction by reducing the activation energy.


Biology- enzyme coursework


Repeat steps for the other temperatures. I will put the 6 different concentrated solutions in a conical flask which will be placed in a water bath. I will work out the gradient of these new results and plot them on a new rate of reaction graph. I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test. We will take you through the osmosis and passive transport, the meaning of words such as hypertonic, isotonic and hypotonic. The method which I used to do this can be seen below. We partner with Say Media to deliver ad campaigns on our sites. Investigate the effect of substrate concentration on the rate of activity of the enzyme catalase. The volume of gas escaping coming from the delivery tube to the burette which will result in an inaccurate reading. Hence it binds to the enzyme active site. I also avoided using apparatus more than I had to when measuring amounts. Passionate about Writing. I felt that this would give me more reliable results in my main investigation because the length of time that the hydrogen peroxide is out of the water bath is reduced. This is important because the more catalyse used the faster the rate of reaction hence the concentration of the catalase and the volume of catalase used for each concentration is to remain the same. Table of Contents. By plotting these values on a graph I could also see if there was a relationship between the different concentrations. They will guarantee you absolute privacy and originality of your work.


Biology- enzyme coursework - GCSE Science - Marked by essayronun.com


The graph above shows that as the H2O2 concentration increases, the rate of reaction also increases. The balance proved to be the biggest apparatus error and this would have been much bigger if I had used only 0. I will then work out the gradient of the two concentrations and plot them on a rate of reaction graph along with the other concentrations. The lock and key analogy… The lock is the enzyme and its active sight is where you put the key in. This is important because if I weigh the yeast and then grind it with the pestle, some of the yeast will be lost because it might get stuck to the pestle, hence slightly decreasing the mass of yeast. This is due to the fact that the catalase is breaking down the substrate, H2O2. Recording Results I will record my results in a table like the one below, and then record further, average results, in a similar table. The gas syringe, however, has a small volume of air displaced within it when it is attached to the conical flask, so I will have to consider this in the main procedure. Do this first to ensure that the mixtures have enough time to reach a constant temperature instead of putting them in for a short time. I predict that as the substrate concentration, the hydrogen peroxide concentration increases, the rate of reaction will also gradually increase. There are two main types of inhibitors: reversible and non-reversible. Here are the proposed solutions to the problems I identified. Natural Sciences, Mathematics, and Engineering Life Sciences The working of the brain like most of nature is all about synchrony. Also, because the hydrogen peroxide had to be inserted into the syringe before the reaction could begin, the amount of time it would be out of the water bath which I intend to use in my main experiment was longer than necessary. Also as the enzyme alters the shape, the enzyme puts on the substrate molecule as result of this it lowers the activation energy. This is because I did not use specific equipment which would have maintained the temperature, which would have allowed me to gain more accurate and reliable result. It catalyses the decomposition of hydrogen peroxide into water and oxygen. Introduction Catalase is an enzyme which is found in most living organisms. They will guarantee you absolute privacy and originality of your work. Trade e. I also used two different methods in order to determine which would be the most effective in gaining the best possible results with minimal error. In other words, since there were fewer molecules initially, this resulted in a lower probability that the molecules would collide. There will be a smaller percentage error due to a large scale and also the oxygen gas will not escape. Biology Coursework Aim To investigate how starch concentration affects an amylase-controlled reaction. Introduction An enzyme is a protein with a special. A2 biology coursework ideas watch. A few years back, someone at my school decided to grow bacteria cultures from samples around the school. The worst thing.


This is used for a registered author who enrolls in the HubPages Earnings program and requests to be paid via PayPal. This is important because if I weigh the yeast and then grind it with the pestle, some of the yeast will be lost because it might get stuck to the pestle, hence slightly decreasing the mass of yeast. Whichever level of education you are in, be it high school, college or university, we are ready to assist you. This shows that the percentage error of the burette is 10 times less than the percentage error of the measuring cylinder. You can Trust us.. By doing this, it will ensure that the test is as fair as I can make it. If the collision causes a chemical change it is referred to as a fruitful collision. However, in my next preliminary experiment, I decided this would be unfair in the main procedure. Passionate about Writing. Determine the optimum temperature. There are also some enzymes which work in an acidic condition such as protease pepsin, which is found in the acidic conditions of the stomach. Overall, there is a pattern showing a constant trend in that as the concentration decreases the rate of reaction decreases too, and that the overall volume of gas evolved also decreases. As result of this I will be using a burette to collect the volume of oxygen as a burette will provide more accurate results. This website uses cookies As a user in the EEA, your approval is needed on a few things. This would show that the reaction is a first order reaction. I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. So therefore pH change alters the shape of the enzyme resulting in the enzyme being denatured as hydrogen and ionic bonds broken. Abstract Help. The faster the particles are going, the more energy they have. This is because the time taken to cover the boiling tube and starting the stopwatch allowed the oxygen to escape due to the fact that the reaction had started as catalase had begun to break down hydrogen peroxide.


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